The aim of this study was to identify the Complex Vertebral Malformation (CVM) in repeat breeder and anoestrous animals in Maharashtra state region. Total 102 animals of HF crossbred, Dangi, Gaolao and Non- descript were screened from field and cattle dairy farm. CVM is a hereditary recessive disorder in Holstein cattle which have a significant economic impact on dairy cattle breeding worldwide. Fifty repeat breeder and fifty-two anoestrous animals blood DNA were extracted by traditional method. Genotyping was performed by using PCR-RFLP analysis with the help of PstI restriction enzyme. In the performed study all animals were normal for Complex Vertebral Malformation. | Study of complex vertebral malformation in repeat breeder and anoestrous cows
