Mature black spear grass (Heteropogon contortus) hydrolysate (SGH) was used for the first time as lignocellulosic substrate for production of bacterial nanocellulose (BC) by Gluconacetobacter xylinum at 30oC and pH . The maximum total reducing sugar (TRS) from SGH was using 6% H2SO4 at 121oC for 4h with higher amount of xylose than glucose and arabinose. BC yield was comparable to those from reported lignocellulosic hydrolysates. | Bacterial cellulose production from mature black spear grass hydrolysate by gluconacetobacter xylinus Effect of pH fermentation time and nitrogen supplementation