Johne’s disease (JD) is caused by Mycobacterium avium Subsp. Paratuberculosis (MAP) and the disease is characterized by chronic diarrhea, progressive weight loss and emaciation. The present study was carried out to find out the MAP infection status by postmortem and molecular diagnosis in sheep and goats suspected for JD. | Pathological and molecular diagnosis of paratuberculosis in sheep and goats