Free living diazotrophic bacteria bacteria were isolated from the rhizosphere soil of two cultivated rice varieties grown in R. S. Pura and Bishnah blocks of Jammu district of Jammu Division of J&K State. Soil samples were collected from rice rhizosphere at panicle initiation stage. A total of twenty rice grown villages were selected and from each village two composite samples were taken from five plants one each from variety Basmati370 and Ratna through random sampling from each rice growing village of R. S. Pura and Bishnah blocks of Jammu district by using GPS. | Enumeration isolation and identification of diazotrophic bacteria in rhizosphere soil of two rice varieties in Jammu district India