A simple, rapid and sensitive ultrahigh performance liquid chromatographic method was developed for the determi‑ nation of the anti-diabetic drug: Empagliflozin (EMPA) and three related substances in spiked human plasma, using dapagliflozin (DAPA) as an internal standard and tetrahydrofuran as a plasma protein precipitating agent. | A UPLC DAD method for simultaneous determination of empaglifozin and three related substances in spiked human plasma