In the present study two diferent RSLC columns, Acclaim RSLC 120 C18, µm, ×150 mm (column A) and Acclaim RSLC 120 C18, µm, ×100 mm (Column B) were utilized for the analysis of velpatasvir (VPS) in presence of sofosbuvir (SFV), where due to the encountered fluorescent properties of VPS fluorescent detection at 405 nm after excitation at 340 nm (Method 1) was used for its detection where the non-fluorescent SFV did not interfere. | Two novel UPLC methods utilizing two diferent analytical columns and different detection approaches for the simultaneous analysis of velpatasvir and sofosbuvir Application to their co-formulated tablet
