Flickingeria fimbriata is commonly applied in China as a traditional Chinese medicine (TCM), however the quality control of it is incomplete. In this work, we aim to identify and quantify the structures of C-glycosyl flavones in F. fimbriata. | Identification of C-glycosyl flavones by high performance liquid chromatography electrospray ionization mass spectrometry and quantification of five main C-glycosyl flavones in Flickingeria fimbriata