The present study was conducted to evaluate the effect of Poly-3-Hydroxy butyrate against the sporulation of oocyst of E. tenella. In this study four different doses of PHB which was extracted from the Bacillus subtilis culture, 10, 20, 50and 100mg were used with potassium dichromate as positive control and distilled water as negative control. The experiment was carried out in 24 well plates. | A preliminary in vitro study to evaluate Poly-3-Hydroxy butyrate as an anticoccidial agent against oocysts of E. tenella