Suitable medium for rooting was MS + mg/L IBA with root shoots at , average roots at roots/shoot after 4 weeks. On organic substrat, 70% coconut fiber and 30% composted cow manure gave the highest survival rate of . The plants grew and developed well during the nursery stage. | Study on in vitro propagation of Giao co lam Gynostemma pentaphyllium Thunb.