The TLC fingerprints of all three medicinal plants had the same visual ability when the fingerprint detected by UV (λ = 254 nm) and the reagent containing g vanillin in 28 mL of methanol: 1 mL of sulfuric acid. In brief, the three active compounds including citral (Citrus aurantifolia), rhdomyrtone (Rhodomyrtus tomentosa) and catechin hydrate (Camellia sinensis) contained in the extract of 3 medicinal herbs had the limited detection (LOD) at 195 ng/spot, ng/spot and 625 ng/spot, respectively. | Separation of active constituents in some medicinal plants by thin layer chromatography