The aim of this study was to analyze the extended spectrum of β lactamase (ESBL), metallo β lactamase (MBL) and AmpC production in Pseudomonas aeruginosa in various clinical samples. A Total of 100 clinical isolates of P. aeruginosa were collected from different clinical specimen and confirmed by standard tests. Antibiotic susceptibility was determined by the Kirby-Bauer disc diffusion method. ESBL screening was done using 3rd generation cephalosporins and confirmatory combined double disc test, imipenem-EDTA double disc synergy test for MBL enzyme and AmpC test using Cefoxitin disc. | Prevalence and antibiotic resistant pattern of Pseudomonas aeruginosa at a tertiary care centre of north India