Twenty isolates of Sclerotium rolfsii Sacc. collected from different hosts and locations of India was studied in relation to genomic DNA amplification through internal transcribed spacer (ITS-PCR) analysis. These isolates of S. rolfsii showed variation at rDNA level which was revealed through 4 primer series. | Genotypic diversity among indian isolates of sclerotium rolfsii Sacc. Teleomorph Athelia rolfsii Curzi Tu Kimbrough based on ITS region of ribosomal DNA