Present study was undertaken to evaluate the anti-apoptotic effect of LEHD-FMK (caspase inhibitor) on buffalo bull sperm before and after semen freezing. Tris egg yolk extender was treated with Z-LEHD-FMK (0, 2, 4, 6, 10 and 20 µM) and used for extension of six aliquots of semen from each ejaculate to final concentration of 80 million sperm/ml. Sperm samples were analyzed for sperm motility, viability, plasma membrane integrity, mitochondrial membrane potential and PLA (Phospholipase) activity at pre-freeze and post thaw stages. | Minimization of apoptosis like changes developed during cryopreservation of buffalo bull sperm by supplementing Z-LEHD-FMK Caspase Inhibitor
