The present investigation was carried out on thirty six genotypes of tomato for genetic variability assessment. The analysis of variance revealed that significant genetic differences were present among the tomato genotypes for all the traits studied. High GCV and PCV estimates were observed for number of fruits per plant, average fruit weight, fruit yield per hectare, fruit yield per plant, number of flowers per cluster and number of fruits per cluster. Number of fruits per plant recorded maximum heritability followed by average fruit weight, number of flowers per cluster, number of fruits per cluster, fruit length, days to first fruit ripening, fruit yield per plant, fruit yield per hectare, fruit width, 100 seed weight and days to first fruit set. | Estimation of genetic variability for yield and yield related traits in tomato Solanum lycopersicum L. under polyhouse condition