Rust caused by Puccinia arachidis Speg. is the most important disease on Groundnut worldwide. Limited information about nature of inheritance of rust resistance is available to peanut breeders. The investigation was undertaken to determine the mode of genetic inheritance of rust resistance in two F2 populations of groundnut. The crosses were generated by crossing resistant genotypes viz., GPBD5 and ICGV86699 with susceptible genotype TG76. The parents, F1’s and F2 populations were evaluated for rust resistance under field conditions by following spreader row technique and spraying uredinospore suspension at 30 and 40 DAS. | Inheritance of rust resistance in groundnut Arachis hypogaea L.