In the present study, new CPV-2a field strain (KLD3) isolated in cell culture was selected and the whole CPV VP2 gene was used for the expression in the E. coli expression system. Prokaryote expressing monocistronic DNA cassette containing open reading frame of whole CPV capsid gene (VP2) downstream of T7 promoter was synthesized. Analysis of the expression in E. coli cells showed the presence of capsid protein. Recombinant capsid protein showed immunoreactivity similar to the whole CPV virus antigen, when reacted with polyclonal antibodies against the whole CPV virus particles. The use of indigenously developed recombinant protein, being very economical, can be used to develop field kit. As the recombinant protein is not infectious, use of it for CPV serodiagnostic assay is considered safe. | Cloning and expression of recombinant VP2 capsid protein gene of canine parvovirus in E. coli System
