Brahmi (Bacopa monnieri L.) belonging to the family Scrophulariaceae is an endangered perennial, glaburous, succulent creeping herb of great medicinal importance. Increased demand of Bacopa based drugs makes natural populations of this herb vulnerable to overexploitation. It is therefore essential to optimize and scale up in vitro multiplication protocols for conservation and multiplication of wild and cultivated germplasm. Nodal segments with axillary buds of an elite Bacopa genotype, were surface sterilized and inoculated to initiate aseptic cultures on per cent agar semi-solidified MS medium ( g/l) containing 3 per cent sucrose at pH . For initiating cultures medium was supplemented with different concentrations of BAP ( mg/l) and kinetin ( mg/l). | Scaling up of protocol for in vitro multiplication and conservation of elite genotype of Bacopa monnieri L. Pennell
