Whey, a by-product of cheese, paneer or casein production, is a high value byproduct of the dairy industry due to its nutritional composition and availability in large volumes. Owing to its high BOD (Biological oxygen demand) value and availability in surplus quantities, whey disposal is a serious problem from both an economical and an environmental point of view. Keeping these facts in view a study was conducted to assess the suitability of paneer whey as a growth medium of L. acidophilus for the production of inhibitory substances against E. coli, S. aureus, to optimize the production conditions and to partially characterize the antimicrobial agent present in the whey fermentate. On using sterilized paneer whey as the growth medium highest antimicrobial activity was obtained upon incubation at 370C for 72 hours. | Paneer whey as a growth medium for the production of antimicrobial agents by Lactobacillus acidophilus - Optimization of production conditions