Lipopolysaccharide (LPS) is the major outer membrane component of the Gram negative bacteria, supporting the structural integrity and protecting the membrane from chemical attack. Lipopolysaccharide is pyrogenic, causes endotoxic shock and induces the clinical manifestations of infections. It has the broad applications in cell stimulation experiments which abstract, the basis for studies on the isolation, purification and characterization of LPS. | Evaluation of two different lipopolysaccharide extraction methods for purity and functionality of LPS