Mechanical ventilation is a life-saving procedure, but it is associated with a high risk of acquiring respiratory infections. Hence to prevent it and initiate empirical antimicrobial therapy, knowledge of microbial profile and their local antimicrobial sensitivity pattern are essential. A prospective analytical study of Endotracheal tube (ETT) aspirates of mechanically ventilated patients in Intensive Care Unit (ICU) was done over a period of six months (Dec2015-May2016). Endotracheal tube aspirates of patients who had been incubated for ≥48hrs were processed by standard methods with the aim to identify the microbiological profile of ETT aspirates and their antibiogram. Out of the 47 samples, were culture positive. Gram negative enteric aerobic bacteria were isolated from most of the patients, most common being Klebsiella species (), followed by Acinetobacter and Pseudomonas. | Microbiological profile and antimicrobial sensitivity pattern of endotracheal tube aspirates of patients in ICU of a tertiary care hospital in Bhopal India