The present study is on the isolation, characterization and screening of endophytic bacterium ., Gluconacetobacter diazotrophicus which was isolated from the tissues of surface sterilized roots of four different crops viz., sugarcane, maize, pineapple and carrot. The morphological and biochemical tests permitted characterization of Gluconacetobacter diazotrophicus isolates. Further the plant growth promoting traits such as nitrogen fixing ability, production of phytohormones (Indole acetic acid and Gibberellic acid), phosphorus and zinc solubilization and antagonistic activity by dual culture method were assessed. Out of 113 isolated screened only 10 efficient isolates from various crops were selected based on nitrogen fixing capacity. | Isolation and screening of nitrogen fixing endophytic bacterium Gluconacetobacter diazotrophicus GdS25