This study attempted to determine the prevalence of HBV infection and comparative assessment between serological and molecular diagnoses among 75 samples divided into three groups as treated, untreated and control groups from different cities in Wasit Province, Iraq, during the period from March 2016 to May 2016. The samples were analyzed by ELISA and Real Time – PCR techniques for detecting the prevalence of HBV in patients. | Comparative assessment between serological and molecular diagnosis for patients groups with hepatitis B virus