Understanding ancillary traits related to flower biology is crucial to understand the appropriate time for emasculation and pollination in order to achieve a successful cross to develop hybrids with high yield and other economically important traits. Being an underutilized crop, it has not received all the attention as received for other pulses, hence an investigation was carried out at University of Agricultural Sciences Bengaluru in kharif season of 2015 for the first time in India to study to standardize the time of emasculation and pollination of different varieties and mutants of variety SB-42. From this study, it was observed that the average interval between flower bud initiation and anthesis days was days. Therefore flower buds intended for hybridization should be 2 days old from bud initiation. Pollination timing for making cross will play an important role in seed set so with this view pollen viability and stigma receptivity has been studied and found that best time for making cross is 1 . to 3 . The results of the present investigation explored the opportunities of utilizing information in order to develop hybrids with multiple desired characters. | Studies on emasculation and pollination in underutilized legume-bambara groundnut- Vigna subterranea L. Verdc.