Streptococcus pneumoniae is the most common cause of community-acquired respiratory tract infections and one of the common causes of morbidity and mortality in developing countries. Penicillin has been the drug of choice for treatment of pneumococcal infections but there is increasing number of reports of penicillin resistant pneumococci (PRP) reported throughout the world. There is also an increase in multidrug-resistant strains. However, there are very less reports available from India. Aims: The aim of the study is to isolate and identify Streptococccus pneumoniae in clinical samples and to screen for penicillin resistance and study the antibiotic resistance pattern. S. pneumoniae was identified with Gram staining, bile solubility, inulin fermentation and sensitivity to optochin as per standard technique. | Study of penicillin resistance and multidrug resistance of streptococcus pneumoniae in a tertiary care hospital