The present experiment aimed to evaluate variability, heritability and genetic advance as per cent over mean for physiological and biochemical traits in twenty one thermo tolerant tomato genotypes. The experiment was conducted at the Experimental Farm, Division of Vegetable Science, Indian Agricultural Research Institute, New Delhi during spring– summer season (March-June) of the year 2014. | Evaluation of genetic parameters for physiological and biochemical traits in tomato Solanum lycopersicum L.