The aim of the present study was to determine the distribution of pathogens and their antibiotic profile against the commonly used antibiotic therapy. A total of 5614 blood specimens from patients were received from intensive care units in Villupuram Medical College, Tamilnadu, India, were cultured by standard microbiological procedures for the isolation of pathogens. The isolates were subjected to antimicrobial susceptibility assay to investigate the resistance pattern of isolates by disc diffusion method and the reports were analyzed retrospectively over the period of three years from Jan 2013 to Dec 2015. | Frequency of bacterial pathogens and antibacterial susceptibility pattern of isolates from blood culture A retrospective study from a tertiary care hospital