To manage the disease an investigation at the Department of Plant Pathology, SHIATS-DU, Allahabad was carried out to evaluate the effect of bio-agents viz. Trichoderma viride @ 2%, Pseudomonas fluorescens @ 2%, T. harzianum @ 2%, botanical viz. neem leaf extract @ 5%, neem oil @ 5% and fungicide viz. carbendazim @ against R. solani under invitro condition by dual culture and poisoned food technique. All the bioagents and botanical were evaluated singly. All the treatments significantly inhibited the mycelial growth of R. solani as compared to untreated check. Maximum inhibition per cent of mycelia growth of Rhizoctonia solani was recorded in Trichoderma viride () followed by neem oil (), P. fluorescens (), T. harzianum () and neem leaf extract () as compared to treated check (Carbendazim) () and untreated check (0%). | In vitro effect of bio- control agents and selected botanical against root rot Rhizoctonia solani Kuhn of Chilli Capsicum annuum L.