Comparison between aquaponics, hydroponics and traditional method for cultivation of spinach was carried out. In this study, spinach was grown in the soilless media like perlite and sphagnum moss. Medias were used to support plant growth. From these Media the perlite media was used in aquaponics and sphagnum moss was used in hydroponics. This study was carried out to examine different morphological characters like height, germination period, surface area, yield of spinach, and biochemical analysis like protein, carbohydrate, chlorophyll content. | Comparative cultivation and biochemical analysis of Spinacia oleraceae grown in aquaponics hydroponics and field conditions