Canine parvovirus (CPV) is a highly contagious cause of serious and often fatal disease in dogs. An unvaccinated male non-descriptive pup of 4 months old with the history of pyrexia, frequent haemorrhagic diarrhea and vomiting was presented to the Infectious Unit of Teaching Veterinary Clinical Complex (TVCC), Veterinary College and Research Institute, Namakkal. The CPV infection was confirmed by polymerase chain reaction (PCR) with concurrent infection by Ancylostoma sp and complication by . However, the pup responded to the treatment positively with suitable antibiotics, anthelmintics and supportive therapy. | Incidence of canine parvo Virus-2 with ancylostomosis and complication by E. coli in a young pup