An investigation was carried out at the Department of Floriculture and Landscaping, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore during 2014-2016 on the improvement of tuberose var. Prajwal through mutation breeding. The bulbs were treated with gamma rays, diethyl sulphate (DES) and ethyl methane sulphonate (EMS). The treatments consisted of , , , and kR of gamma rays, 15, 20, 25 and 30 mM of DES and 30, 45, 60 and 75 mM of EMS and control (untreated). Various morphological and floral characters were observed. In general, the treated population had manifested reduced expression than the control (untreated population) for most of the morphological and floral characters. Higher the dose of mutagens, lower was the expressivity of the traits. Expression of the morphological characters namely plant height, number of leaves, leaf length, leaf width and leaf thickness increased in the lower doses and decreased in the higher doses in M1V2 generation. | Effect of physical and chemical mutagens on morphological characters in M1V2 generation of tuberose Polianthes tuberosa L.