Ashwagandha (Withania somnifera (L.) Dunal) is a potent medicinal plant originated in the Indian sub continent and is used in traditional systems of medicine globally. Pure lines form an important genetic resource for improvement of yield and quality. In the present study, a set of 327 (DWS1-DWS327) pure lines were developed from JA134 out crossed population through individual plant selection, selfing and generation advancement for the first time in Ashwagandha. Variation for qualitative and quantitative traits was observed between pure lines and lines with distinct morphological traits were obtained. Heritability and genetic divergence among a set of 48 pure lines with JA134 and JA30 was assessed based on 20 root yield and its component traits. | Genetic diversity for root yield and its component traits in Ashwagandha Withania somnifera L Dunal pure lines derived from JA134 population