Aquaculture products can harbor pathogenic bacteria which are part of the natural microflora of the environment. Feeding infected fish with antibiotic- medicated food is a general practice but has led to antibiotic resistance development in bacterial pathogen, resulting in a higher dose requirement for effective control, a matter of increasing public concern. Resistance of pathogens to antibiotics occurs due to the presence of β-lactamase enzyme, thereby resulting in Multiple Drug Resistance (MDR). These enzymes are present in Enterobacteriaceae such as E. coli. | Isolation and PCR amplification of E. coli from freshwater fish Cirrhinus cirrhosis and its PCR amplification of SHV gene