Sorghum bicolor is most important cereal crops around the world where the availability of water is less. Study of genetic variability among the sorghum accessions will enable accurate results in breeding programme and in mapping studies. By using SSR markers evenly distributed on sorghum genome revealed that, all the 20 accessions originated from Marathwada (Maharashtra) region were grouped into three main classes and it show correlation with the biological status and other characteristics features of accessions. | Analysis of genetic diversity in sorghum Sorghum bicolor L. accessions of Maharashtra as estimated by simple sequence repeats SSR