Antifungal metabolites from rare actinomycetes have been found attractive for application due to its novelty, potency and environmental friendliness. Optimization of process variables for enhanced production of antifungal metabolite in Janibacter sp. RC18 was carried out in this study. One factor at a time (OFAT) was used for preliminary optimization of fermentation variables (time, temperature, initial pH, carbon and nitrogen sources). | Optimization of nutritional variables using response surface methodology for enhanced antifungal metabolite production by Janibacter sp. RC18 from turmeric Rhizosphere