Bitter gourd (Momordica charantiaL.) is an important cucurbitaceous plant in which the deleterious diseases namely vascular wilt, damping off occurs commonly and causes 30 to 50 percent losses in crop. The present investigation was aimed to study the morphological and molecular characterization of disease causing pathogen to understand the etiology of the disease. Survey was conducted at different locations of Coimbatore, Erode and Dharmapuri and samples were collected for the isolation of the pathogen. The pathogen was isolated from the brownish discoloured vascular tissue of stem portion which was typically wilted. Pathogenicity assay was proved and the virulence of the fusarium isolates were identified under artificial inoculation studies. | Morphological characterization and genetic diversity of Fusarium spp. infecting bitter gourd