The study measured and compared quality parameters such as pH, moisture content, ash content and hydrogen peroxide concentration of three types of honey from different localities in Ghana. Using the agar well diffusion, antimicrobial potential of natural honey from two different sources as well as an adulterated honey were determined against Staphylococcus aureus (ATCC 25923), Escherichia coli(ATCC 35218)and Klebsiella pneumonia (ATCC 27736). The results of the physico-chemical properties showed the following range of values; pH of (– ), moisture content ( – ) and ash content ( – ). The moisture content of the adulterated samples at 25% and 50% concentration in all cases exceeded the recommended 21%of good quality honey. | A comparative study on the antimicrobial activity of natural and artificial Adulterated Honey produced in some localities in Ghana