The SSR technique was used to reveal the genetic diversity among wheat (Triticum aestivum) and its wild relatives and secondly to test the hybridity of wheat (Triticum aestivum) F1s. All the thirteen SSR primers showed 100% polymorphism and except one, all the primers generated unique bands, so these primers can be used for genotype identification. Also four primers showed heterozygous nature of F1s by giving two bands, one from each parent at a particular locus, so these markers can be used for screening purpose also. Use of these primers resulted in 180 polymorphic bands out of which 47 were unique. | The potential of SSR Markers to reveal the genetic diversity among wheat and its wild relatives and to test the hybridity of F1s