In the present study ten different Bacillus isolates were isolated from various soil samples and their identification was performed on the basis of Gram staining, colony morphology and biochemical tests. Gram positive rod shaped bacteria showing large, circular, opaque colonies having white or greyish white appearance were preserved on Nutrient agar slants for further identification. All isolates showed positive results for Catalase, Urease, Oxidase and motility biochemical tests. For other biochemical tests such as Citrate utilization, Vogues Proskauer, Nitrate reduction, Triple sugar iron Agar test, mixed results were obtained. Phosphate solubilisation and Potassium solubilisation activities of different isolates was performed. Out of ten isolates, none of the isolates solubilised phosphate on Pikovskaya’s agar medium at 30°C after 15 days of incubation. | Isolation and identification of Bacillus species from soil for phosphate potassium solubilisation and amylase production
