The presence of anti-nutritional factors in jatropha kernel meal such as, phorbol esters, lectins, trypsin inhibitor, phytate and saponins is of great concern. Toxicity of meal is mainly due to the presence of phorbol esters which limits its use. Several methods have been tried for detoxifying kernel meal that includes physical, chemical, biological and radiation methods. In the study, four different samples, ., raw, defatted, one-time mechanically oil expressed and two-times mechanically oil expressed samples were prepared from jatropha kernels. | Phorbol ester degradation using biological treatment in Jatropha kernel meal