The present investigation was carried out with an aim to isolate and characterize native Bacillus thuringiensis from Anand district of Middle Gujarat. Total 67 probable B. thuringiensis were isolated from 17 diverse soil samples, out of which 9 isolates were selected based on presence of parasporal crystal body and primary toxicity test. Selected nine native isolates were characterized based on their morpho-cultural and biochemical characteristics. | Isolation and insecticidal potential of native Bacillus thuringiensis against Helicoverpa armigera and Spodoptera litura