The experiment was incepted to understand the effect of bio fertilizers on enhancement of physical properties of Agaricus bisporus. The application of bio fertilizers at the time of casing gave slightly better result followed by application of bio fertilizers at the time of spawning. All the treatments of bio fertilizers . Pseudomonas putida and PSB (Bacillus megaterium) either alone or in combination were found significantly effective in improving growth and yield of A. bisporus. | Effect of bio-fertilizers on mycelial growth and physical properties of white button mushroom Agaricus bisporus Lange Imbach