Panchamirtham is a kind of fruit based fermented product with a strong flavour. Panchamirtham is made from the five types of foods were used in Hindu worship and pooja to divinity. The current study was conducted to investigate the diversity of native lactic acid bacteria (LAB) populations and characterization in the indigenous fermented fruit mix. Initially, seven hundred and eighty seven isolates were enumerated from fermented fruit mix from five different media such as Eosin Methylene Blue, Lysogeny agar, Nutrient Agar, R2A and Endo Agar. | Characterization of probiotic bacteria from fermented fruit mix