Non-fermenting gram negative bacilli (NFGNB) are known to account for nearly 12- 16% all bacterial isolates from a clinical microbiology laboratory. The most common infections caused by these organisms were septicemia, pneumonia, urinary tract infections, surgical site infections, wound infections, osteomyelitis, etc. As multidrug resistances being very common and increasing among NFGNB and Pseudomonas and Acinetobacter being the most predominantly isolated NFGNB and its resistance towards colistin and imepenam type of antimicrobials is of major concern. To isolate, identify and characterize the prevalence of NFGNB along with their antimicrobial sensitivity pattern among the patients attending a tertiary care centre in Tamilnadu. A prospective study was conducted in our hospital for a period of two years from Jan2012 to Dec 2013. | Prevalence of non-fermenting gram negative bacilli and their antibiotic sensitivity pattern at a tertiary care hospital in Tamilnadu India