A total of sixty isolates were obtained from rhizosphere, nodule and phyllosphere of different crops. These isolates were analysed for some of their plant growth promoting traits like IAA production, phosphate solubilisation, siderophore production, HCN production, ACC utilization and ammonia excretion. Apart from these traits all the isolates were also screened for their growth at different temperatures and salt concentrations. Three promising isolates (MP12, PP1 and UP4) were characterized morphologically, biochemically and on the basis of 16S rRNA gene sequencing. Phylogenetic tree of methanol utilizing isolates MP12, PP1 and UP4 was prepared by using neighbour joining of BLAST programme. | Isolation and characterization of methanol utilizing microorganisms from leguminous crops
