The analysis of abiotic stress responsiveness in plants is a very crucial way to identify the genes conferring stress tolerance and their use in crop improvement programme. Heat stress is one of the stresses which highly affect the growth, quality character as well as total yield of the crop. In this study, a proteome analysis by 2D electrophoresis was used to identify the characteristic change in two wheat genotypes viz, GW 451 (heat tolerant) and WH 147 (heat susceptible) from flag leaf. Control and treated leaf samples showed the expression of differentially expressed proteins at booting stage in both genotypes of wheat. After the completion of 2DE, expressed protein were analysed as spot formation on gel. The comparison of two different gel as control and treated for each genotypes gave the information regarding molecular weight, pH, volume of that protein etc. | Proteomic analysis in wheat to study the effect of heat stress on flag leaf
