Fucoidan is a biologically active polysaccharide found in brown seaweeds. The present study was carried out to isolate and investigate the biological activities of fucoidan and the activity of fucoidanase (EC: ) from Padina pavonica, Sargassum latifolium and Colpomenia sinuosa. C. sinuosa expressed the highest fucoidan content and the highest fucoidanase activity. | Fucoidan and Fucoidanase from brown seaweeds and applications