Transmission electron microscopy (TEM) is a microscopic technique in which a beam of electrons is transmitted through an ultra-thin specimen, interacting with the specimen as it passes through. An image is formed by the interaction of the electrons transmitted through the specimen; the image is magnified and focused onto an imaging device, such as a fluorescent screen or on a layer of photographic film, or to be detected by a sensor such as a CCD camera. | Uses of transmission electron microscope in microscopy and its advantages and disadvantages