Mycoplasma gallisepticum and Mycoplasma synoviae are important poultry pathogens responsible for high economic losses to the poultry industry in term of mortality, poor weight gain and decrease of feed efficiency in broiler chickens and reduction of egg production in layer chickens and turkeys. Serum plate agglutination (SPA), hemagglutination inhibition (HI) and ELISA are the most common serological techniques used for rapid detection of infection and the adoption of control measures of the diseases. Application of molecular techniques like PCR, real time PCR is currently used for the demonstration of the DNA of the pathogen. | Prevalence of Mycoplasma gallisepticum and Mycoplasma synovae in Poultry - India perspective