Antimicrobial activity and phytochemical screening of neem leaves and lemon grass essential oil extracts

In this study, the antimicrobial activity and phytochemical constituents of neem leaves and lemon grass oil extracts were evaluated. Oil extracts of neem leaves and lemon grass were obtained by solvent extraction method using hexane and ethanol. | Antimicrobial activity and phytochemical screening of neem leaves and lemon grass essential oil extracts International Journal of Mechanical Engineering and Technology IJMET Volume 10 Issue 03 March 2019 pp. 882-889. Article ID IJMET_10_03_091 Available online at http ijmet JType IJMET amp VType 10 amp IType 3 ISSN Print 0976-6340 and ISSN Online 0976-6359 IAEME Publication Scopus Indexed ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF NEEM LEAVES AND LEMON GRASS ESSENTIAL OIL EXTRACTS Damilola Elizabeth Babatunde Grace Oluwatomisin Otusemade Modupe Elizabeth Ojewumi Oluranti Agboola and Esther Oyeniyi Department of Chemical Engineering Covenant University PMB 1023 Ota Ogun State Nigeria Kehinde Deborah Akinlabu Department of Biochemistry Covenant University PMB 1023 Ota Ogun State Nigeria ABSTRACT In this study the antimicrobial activity and phytochemical constituents of neem leaves and lemon grass oil extracts were evaluated. Oil extracts of neem leaves and lemon grass were obtained by solvent extraction method using hexane and ethanol. Antimicrobial activity screening of plants oil extracts were conducted using agar well diffusion method and the oil extracts were tested against three gram negative bacteria Pseudomonas aeruginosa Klebsiella specie Escherichia coli one gram positive bacteria Staphylococcus aureus and two fungi Candida albicans Rhizopus specie . Phytochemical components of the ethanolic oil extracts were anthocyanin and betacyanin quinones terpenoids and acid for lemon grass. In addition to other phytochemicals present in lemon grass ethanolic oil extract neem ethanolic oil extracts tested positive to flavonoids. Lemon grass oil extract shows high activity against Escherichia coli Staphylococcus aureus and Candida albicans which are representative of the three categories of microorganisms considered. Neem leaves oil extracts have relatively low activity against most of the selected microorganisms. Keywords microbial

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