Biofilms are surface associated bacterial communities surrounded by a matrix of exopolymers. Biofilms contribute to the development of persistent infections due to their refractiveness to antibiotic therapy. The purpose of this study was to evaluate three methods for detection of biofilm formation in different indwelling devices. | Comparison of phenotypic methods for the detection of biofilm production in indwelling medical devices used in NICU and PICU in a tertiary care hospital in Hyderabad India